15th New Enzymology Kinetics Workshop

January 9-12 2024   Austin, Texas

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modern kinetic analysis 4-day intensive with Dr. Kenneth A. Johnson

Modern kinetic methods coupled with high resolution structural data provide a powerful tool to establish reaction mechanisms. In this intensive four-day course taught by Dr. Kenneth A. Johnson, modern kinetic analysis using a variety kinetic and equilibrium methods will be detailed using numerous examples on proteins and nucleic acids. The course will focus on developing the path from experimental design to data collection and analysis to yield new mechanistic insights.

Over the course of 4 days, you will:

  • Understand the dynamics of enzyme reactions
  • Plan and execute informative kinetic experiments
  • Fit kinetic data to extract mechanistic information
  • Understand the information content of kinetic data
  • Use simulation to predict results
  • Fit data directly to your model
  • Fit pharmacokinetic/dynamic and SPR data
  • Globally fit multiple experiments to a single unifying model

A central feature of the course is the use of KinTek Explorer software to learn kinetics and rigorously fit data. Participants will receive a six-month license for the software (for Mac and Windows) to use during and after the course. Tutorials on the use of computer simulation will develop an intuitive understanding of reaction kinetics, and hands-on problem solving will facilitate integration and mastery of the course material.

A complete schedule and detailed breakdown of program content followed by an online registation form can be found below. This information is also available as a downloadable PDF document.

Workshop Schedule

Hyatt Regency Austin

Program Content

  1. Mechanism-based data fitting. The course will focus on the use of computer simulation based on numerical integration of rate equations obtained from a model entered by the user. The primary goal of the workshop will be to help you to become proficient in modeling and fitting data using KinTek Explorer software so that you can tackle any problem.
  2. Steady state kinetics. The information content of steady state kinetic measurements will be described in discussing the meaning of the kinetic constants, what they tell about a reaction mechanism and what they do not reveal.
  3. Introduction to transient kinetic methods. The need for transient kinetic methods will be described to provide mechanistic information that can be interpreted directly to define reactions occurring at the active sites of enzymes or nucleic acids.
  4. Fundamental principles of reaction kinetics. The basic principles of reaction rate measurement will be described. The simple math behind exponential reaction kinetics will be presented to reveal the information content of kinetic data.
  5. Data fitting principles and practice. The use of computer simulation and nonlinear regression in data fitting will be described. Equations will be presented for general use in data fitting and the meaning of the kinetic parameters will be described. We will show how mechanism-based data fitting overcomes the many limitations of equation-based fitting.
  6. Kinetics of ligand binding. We will begin a discussion of reaction kinetics with the binding of a ligand to a protein or nucleic acid to emphasize the importance of analysis of the concentration dependence of the rate. We will also discuss the relationship between the binding rate constant and the steady state kinetic parameter k cat /K m and the principles governing enzyme efficiency and specificity.
  7. Kinetics of multi-step reactions. The kinetics of two-step reactions will be described under different scenarios. The principles that govern the design of experiments and the modeling of data to distinguish alternatives will be discussed.
  8. Kinetics of ligand dissociation. The kinetics of ligand dissociation in competition experiments will be explored. Examples include the use of protein fluorescence and of fluorescently labeled substrates to measure release of an enzyme substrate from either protein or RNA enzymes.
  9. Analysis of chemical-quench-flow data. Chemical-quench-flow experiments are often more difficult to perform, but easier to interpret because the direct measurement of the conversion of substrate to product eliminates ambiguities in the possible interpretations. These experiments anchor the interpretation of fluorescence experiments in globally fitting data.
  10. Single turnover kinetic studies. The best experiments to look for enzyme intermediates are based upon studies of the conversion of substrate to product with enzyme in excess over limiting substrate. The design criteria for such experiments and their interpretation will be described with examples from several enzymes.
  11. Interpretation of stopped-flow signals. Fluorescence signals are often difficult the origins of the fluorescence change may not be known. These problems are solved by globally fitting data defining the optical changes relative to the individual species in a reaction sequence. We will also describe singular value decomposition methods to analyze time-dependent spectral changes.
  12. Kinetics of slow binding inhibitors. Many potent enzyme inhibitors bind so tightly that at concentrations near their K d the rates of binding are quite slow. Although analysis of slow, tight binding inhibitors is a traditional steady state kinetic problem, the data can best be analyzed by computer simulation.
  13. Single molecule kinetic methods. Observation of single molecules presents several advantages over measurements of ensembles of molecules in bulk solutions but is not without its serious limitations. Our discussion will focus on the relationship between single molecule and ensemble measurements and how the two methods can be used together to gain new mechanistic information.
  14. Global fitting methods. We will show how global data fitting based on computer simulation provides the most rigorous method to extract mechanistic information. This analysis reveals a complete pathway in ways that cannot be obtained by piecemeal equation-based data fitting. In global data fitting we show that the whole is greater than the sum of the parts.

Additional Notes

Participants must bring a laptop in order to develop expertise in the use of KinTek Explorer to simulate kinetics. This powerful new tool provides hands-on experience in relating observable kinetic data to underlying models, enabling researchers to pose questions such as: What would the data look like for a given model? Can a proposed experiment distinguish alternative models? Which rate constants are determined by these data? Moreover, KinTek Explorer Software will be used to illustrate the fitting of data by nonlinear regression directly to a model. Fitting data by simulation directly to a model represents a significant paradigm shift that requires the development of some intuition and insight. In this short course we will work to develop that intuition and teach the skills needed for rigorous data analysis. Please familiarize yourself with KinTek Explorer in advance by downloading the free software, watching video tutorials, and reviewing the manual if you have not done so already.


Use the form below to register and pay securely with your credit card, once for each attendee. Registration is limited and early discounts apply.

Included in the registration fee:

  • Hotel room, 5 nights, check-in 01/08/24, check-out 01/13/24
  • One year license for KinTek Explorer
  • Book: “Kinetic Analysis for the New Enzymology” by K. A. Johnson
  • Morning and afternoon coffee/tea/snack breaks.
  • Banquet dinner on January 12
Cancellations received by 15 November 2023 will receive a full refund. You can also opt to pay a $1000 deposit to secure your spot and then pay by wire transfer or check. Please see registration confirmation email for details.

Industry/Private Sector Academic/Govt Grad Student
Before 1 December 2023 $5970 $2985 $1995
After 1 December 2023 $6480 $3240 $2250
Hotel (Hyatt Regency Austin) is included in the registration fee. Check-in is January 8, 2024 and check-out is January 13, 2024.
Online Registration + Secure Checkout

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